Laparoscopic cholecystectomy is
commonly performed for cholelithiasis. In spite of strict aseptic technique and
preoperative antibiotic prophylaxis, there is risk of port site wound
To study the incidence of positive bile culture and the most common
pathogen involved after laparoscopic
cholecystectomy in patients of cholelithiasis and its correlation with port
site wound infection.
Study design: Cross
Setting and duration: Bahawal Victoria Hospital Bahawalpur, from
01-01-2019 to 31-12-2020.
Method: One hundre patients of cholelithiasis between 18-75
years of age underwent laparoscopic cholecystectomy. Prophylactic antibiotic
was given. Bile with gall bladder was collected and sent for culture. Bacterial
growth was recorded as positive bile culture. Organism type was recorded.
Stone/stones size was also measured.
Data was collected on a proforma which included demographics details
like name, age, gender, BMI, clinical features, size of calculi, culture report
(positive bile culture if some bacteria is isolated and negative no growth) and
type of bacteria isolated and frequency of port site wound infection. Data was
analyzed on SPSS version 21.
Results: The mean age of the patients was 42.44±16.58
years and male to female ratio of the patients was 1:1.8. The mean BMI of the
patients was 25.93±4.67 kg/m2. Bile
culture for bacteria was positive in 30(30%) patients and the most common
organism was E coli 13(43.33%.78%). Positive bile culture was detected in 20
(20%) male patients and 10 female patients. There was significant correlation
between positive bile culture and gender (p value < 0.001). Port site wound
was infected in 6 (6%) patients and in all the patients bile culture was also
Conclusion: Bile culture was positive in 30% patients
of cholelithiasis undergoing laparoscopic cholecystectomy and wound infection
was present in 6% patients and all the
6% patients were bile culture positive .
Keywords: Cholelithiasis, Culture, Organism,