Aim: To establish the diagnostic utility of D2-40 IHC stain in dermatofibroma,
by determining its positivity of expression.
Study design: It was a Cross-sectional study
Setting: The study was conducted in Department of Histopathology, Chughtai Institute
Duration of study: Six months
(1st August 2018 to 31st January 2019)
Methods: A total 70
samples were included. The clinical parameters like age and gender were
recorded. The histological preparation was performed by classic method for
inclusion in paraffin followed by haematoxylin-eosin staining. The
immunohistochemical analysis was performed on serial sections using
immune-enzymatic soluble complex method. The antibody used was D2-40 polyclonal
antibody from DAKO. Diffuse crisp cytoplasmic D2-40 staining was considered
Results: The mean age was 32.76±10.65 years and male to female ratio was 1:1.4.
There were 70% cases have D2-40 positivity for dermatofibroma and 30% have no
D2-40 positivity for dermatofibroma and mean duration of disease was 9.05±6.33
months. There were significant difference (P<0.05) between age and duration
of disease with respect to D2-40 positivity for dermatofibroma;
Conclusion: D2-40 immunoreactivity is sensitive and is useful in the differential
diagnosis of dermatofibroma.
Keywords: Dermatofibroma, Haematoxylin,
Immunohistochemical, Positivity of D2-40