Background: Diffuse large B-cell lymphoma (DLBCL) is a heterogenous neoplasm of
lymphoid series cells constituting 30%–40% of non-Hodgkin lymphoma in adults. DLBCL
presents significant heterogeneity
Aim: To determine the frequency
of activated B-cell subtype of DLBCL by
using Hans algorithm.
Methods: It was designed as a cross sectional study conducted in a duration of
six months (5th Jun 2018 to 7th January 2019). All data
was collected by using a Performa. The clinical parameters like age and gender
were recorded. The histopathological method used was paraffin embedding and hematoxylin–eosin
staining. The immunohistochemical technique used on serial sections was immune-enzymatic soluble complex method.
SPSS version 23 was used for data entry and
analysis. Mean and standard deviation was determined for quantitative variables
like age and tumor size. Calculation of qualitative variables like gender,
tumor site (nodal as well as extra-nodal) and immunohistochemical staining was
done in the form of frequencies and percentages.
The mean age
of the patients was 48.55±15.41 years. Majority of them were male with
frequency of 69(71.9%) and female were
27(28.1%). Mean size of lymphoma was 12.44±4.24cm in the patients. Most
of the cells were nodal with frequency of 58(60.4%) and a few extra nodal with
frequency of 38(39.6%). It was noticed that germinal centre B-cell type was in
53(55.2%) and activated B-cell type lymphoma was present in 43(44.8%).
Conclusion: It is observed that frequency of the activated B–cell type lymphoma are
less common in patients with lymphoma.
Keyword: Lymphoma, Germinal, carcinoma