Background: Asthma is a chronic inflammatory disease affecting smaller airways.
Airflow obstruction leading to airway hyper-responsiveness and increased mucus
production are salient features of asthma pathophysiology. Generally, Th2
cytokines are increased in allergic asthma.
Aim: To propose the molecular mechanisms by which Carica Papaya Leaves Extract (CPLE) alleviates pulmonary edema in
animal model of allergic airway inflammation comparable to methylprednisolone.
Place and duration of study:
Pharmacology Department, University of Health
Sciences Lahore for 1 year.
Methods: We took twenty four male BALB/c mice and divided them equally into four
groups. The control group was given PBS only, while Group II served as diseased
group and induced airway inflammation by ovalbumin. Group III and IV were first
induced with airway inflammation and
side by side treated with Carica
papaya leaf extract (CPLE) 100mg/kg body weight orally and
methylprednisolone 15 mg/kg body weight intraperitoneally for seven consecutive
days respectively. At the end of the experimental protocol, mice were
euthanized and lung wet/dry ratio was measured. mRNA expression of AQP1 and
AQP5 in lung tissue were also determined using RT-PCR.
Results: Ethanolic extract of Carica
Papaya leaves decreased all markers of pulmonary edema in mouse model of
allergic airway inflammation comparable to methylprednisolone by decreasing
lung wet/dry ratio and enhancing AQP1 and AQP5 mRNA expression.
Conclusion: Carica Papaya leaves extract may diminish pulmonary edema in mice associated with
Keywords: AQP1, AQP5 (Aquaporins), Carica
Papaya Leaves Extract (CPLE), Pulmonary Edema, Th2 cytokines.