Aim: To study the comparative effect of Acetaminophen induced
liver toxicity with aqueous extract of Azadirachta Indica (Neem) and vitamin E
on the basis of liver Histopathology.
Methods: Sixty Wistar Rats of both sexes were split into four
groups. Each group contained 15 animals. The control group was group A, Group B
was treated orally with single dose of
Paracetamol 2 mg / kg by weight,
Group C was administrated orally with aqueous Neem extract 500 mg/kg +2
mg/Kg by weight oral Paracetamol, and Group D was given orally paracetamol+Neem
extract+Vitamin E with 100mg/Kg/body weight for 15 days. Rats from all groups
were decapitated, the liver was sliced, and liver tissues were taken for
histological examination. Tissue samples were fixed in 10% formaldehyde,
embedding in paraffin followed by Hematoxylin and Eosin dye (H&E) and
observed under 400x magnification with a digital microscope.
Results: On Histopathological examination of the rat`s liver in we
found that the control group had a normal appearance, colour, and uniform
surface without any necrosis. Group B showed severe necrosis and haemorrhagic
patches. In comparison, Group C revealed normal appearance, colour, and smooth
surface with no necrotic alterations. Livers from the group D looked virtually
normal in terms of colour, undersurfaces, and organ weight. However,
hepatoprotective effects were observed in the Group C and D. Therefore, we can
conclude that Azadirachta indica and Vitamin E could serve as a good medication
for defence against liver injury.
Conclusion: Our findings showed thatextract of Azadirachta Indica and
Vitamin E exhibited hepatoprotective effects on the Wistar rats that were
subjected to Acetaminophen.
Key words: Azadirachta Indica leaf extract, Vitamin E,
hepatoprotective Paracetamol, Wistar rats.