Antimicrobial activity of various fractions from Aloe Vera extract against extended spectrum beta lactamases
Zoobia Nazar Dar, Sidrah Saleem, Usman Arshad, M. Imran, Ayesha Ghazal, Waqas Latif
Aim: To determining the minimum inhibitory concentration and antimicrobial activity of Aloe Vera extract.
Design: A descriptive study.
Place of study & duration: The place of study is University of Health Sciences, Lahore. The duration is one year.
Methodology: The extract of Aloe Vera gel was made in methanol then further its fractions were made in different solvents by solvent-solvent partition and then MIC was determined by double disc diffusion method. I cleaned and entered the data by using SPSS version 16 computer package.
Results: 63 ESBL producing bacterial strains were used in the study and the MICs of each extract were determined. The crude methanol extract gave a minimum inhibitory concentration (64mg/ml) than n-hexane (128mg/ml) and ethyl acetate(64.0mg/ml) and chloroform (256mg/ml) and methanolic fraction (32mg/ml). The study revealed that methanolic fraction and methanol crude extract of Aloe Vera gel was more vulnerable to the ESBLs producing gram negative rods and ethyl acetate, n-hexane and chloroform fractions also showed activity against ESBLs producing gram negative rods and also impart more weight to general adequacy of these extracts for therapeutic determinations.
Conclusion: It was concluded from the study above that all the extracts exhibited encouraging antimicrobial activity against harmful pathogenic organisms.
Key words: Methanol, ethyl acetate, n-hexane, chloroform, ESBLs.