Aim: To evaluate salivary protein-profile and oxidative stress
status in young healthy adult smokers and non-smokers.
Method: Saliva samples were collected from a total of 46
individuals, including non-smokers (n=26) and smokers (n=20) through drooling
method. All participants were males, healthy and aged 18-35 years. Salivary
flow rate and pH was noted. Total salivary protein concentration, oxidative
stress status and amylase activity was measured by spectrophotometric assays.
Salivary protein-profile was evaluated by SDS-PAGE.
Results: Our results showed that salivary pH was significantly
lowered in smokers. Significant increase in levels of reactive oxygen species
(ROS) were found in smokers as compared to non-smokers (p<0.05). Significant
difference was not found in total salivary protein concentration and amylase
activity. However, differences were observed in frequency and intensity of
protein bands. Seven predominant protein bands of molecular weight ranging from
<11-100kDa were observed and frequency of <11kDa protein bands were found
to be higher in smokers.
Conclusion: Increased ROS levels in smokers indicate an oxidative
imbalance in these subjects. Differences observed in salivary protein-profile
needs further investigations centered on identifying whole salivary proteome
which may help in determining early biomarkers for tobacco related oral
Oxidative stress, Protein profile, Cigarette smoke, Reactive Oxygen Species