Background: Cyclophosphamide is a well-known, powerful anti-cancer treatment of
choice for various tumors.
Aim: To study, the protective effect of Berberis Vulgaris methanolic
root extract on hepatocytes of rats during CYP-induction by histopathological
aspect was studied.
Place of Study: Institute of Molecular Biology and Biotechnology (IMBB), The University
Study Design: Observational type of descriptive study
24 adult healthy male albino rats divided in six groups with four rats in each
group (n=4) weighing between 120-200g were contained in Animal House of The
University of Lahore and permissible to saline and usual regime with measured settings
of temperature 25±2 and regular photoperiod (12 hours dark and light) during
the experimentation. Berberis vulgaris root extracts were prepared in 70%
ethanol, filtered and concentrated to dry on rotary evaporator at 50°C and
Cyclophosphamide was obtained from Pharmedic Laboratories (pvt, limited), with
dose of 1000mg/kg and 80mg/kg respectively, prepared in water for injection.
Results: Histological examination of saline treated group showed normal structure
while the negative control group showed necrosis of hepatocytes, vacuole
generation, marked cellular swelling and presence of inflammatory cells. The
rat liver in plant control group A and B showed normal architecture like saline
treated control group with blood vessel congestion. Analysis of prophylactic
group showed slight presence of hepatocyte necrosis, vacuole generation, and
cellular swelling in recovery phase.
Conclusion: Prophylactic group with Berberis vulgaris root extracts 1000
mg/kg exhibited noticeable safety in contradiction of diminished liver
functions and toxicity induced by Cyclophosphamide.
Keywords: Berberis vulgaris, Cyclophosphamide,