the period of October, 25th 2019 and January, 15th
2020, twenty sampleswere collected from different
clinical specimens (stool and urine) from patients attended to the (Rapareen
Padiatric and Hawler Teaching hospitals) in Erbil city. The sample was bringing
to the laboratory and re-identified by performing morphological, cultural,
biochemical tests and confirmed by API 20E systems.The
isolates appeared to be varied in their resistance
to eleven antibiotics; Imipenim (IMP) was the most effective antibiotic against
all isolates of E. coliand all isolates were resistant 100%, to
Ticarcillin (TIC), Cephalothin(CEP) and Amoxycillin+ Clavulonic acid (AMC). The
resistance rate of the isolates for Amikacin (AK) were 10 %, 50% for Nalidixic
acid (NA) and Nitrofurantoin (NIT), 60% for Ceftriaxone (CTR), 65% for Cefotaxime
(CTX), 85% for Piperacilin(PI), 95% for Ampicillin(AMP), respectively. AllE.
coliisolates were screened for the presence of (ChuA) gene on genomic DNA using polymerase
chain reaction (PCR) assay. Results showed that
all isolates were positive for the existence of ChuA virulence gene.