Morphometric Study about Effect of Sleep Deprivation on Prostate of Rats with Protective Effect of Omega 3 Fatty Acids

Abstract

Background: Even though sleep makes up nearly a third of a person's lifetime, we do not fully appreciate its significance. The partial or nearly complete loss of sleep from an organism that results in a number of detrimental health issues is known as sleep deprivation.

Aim: To investigate how omega 3 fatty acids affect the size and weight of the prostate gland in sleep-deprived rats.  

Study design: Random control trial.

Methodology: Thirty male Sprague Dawley rats (population) aged three to four months and weighing 200 to 300 grammes were utilised. They were kept in a controlled setting at the NIH's animal house in Islamabad. Rats were randomised into three groups by using lottery method, with n = 10 rats in each group. The control group was designated as group A. They experienced a regular cycle of sleep and wakefulness.  Rats in group B, which experienced sleep deprivation for 16 hours followed by an 8-hour sleep window each day for 8 weeks, were placed in the sleep-deprived group. The standard lab diet was provided to Groups A and B. For eight weeks, Group C received a typical lab meal combined with omega 3 fatty acids while also being sleep deprived for 16 hours, followed by an 8-hour sleep window each day.

Results: In comparison to groups A and C that received omega 3, the gland weight and volume in group B were significantly lower. Group A: Weight and volume of the prostate: Prostate weight ranged from 1.85 to 0.50 grammes on average. The computed RTBWI was 0.60 + 0.22. The prostate's mean SD volume was 166.81 45.67 mm³. GROUP B: Weight of the prostate: Prostate weight ranged from 1.30 to 0.22 grammes on average. In the sleep-deprived group B compared to the control group A, the weight of the prostate was considerably lower (p-value=0.008). The computed RTBWI was 0.37 + 0.22. The prostate's mean SD volume was 127.88 67.35 mm³. GROUP C: Weight of prostate gland: In experimental group C, the prostate's mean SD weight was 2.19 0.42gm. According to the RTBWI calculation, it was 0.64 0.07. The prostate's mean SD volume was 182.89 44.16 mm³.

Practical Implication: Today, sleep deprivation is a big problem, and this study will assist researchers in examining how Omega 3 fatty acids can help prevent this problem and the harmful effects it has on the prostate glands. 

Conclusion: It was concluded that Omega 3 fatty acids played a protective role while sleep deprivation had a negative impact on the weight and volume of the prostate gland.

Keywords: Sleep Deprivation, Omega 3 Fatty Acids, Prostate and Protective Effect.