Screening of Anticancer and Immunomodulatory Properties of Recombinant pQE-HAS113 Clone Derived from Streptococcus Equi

Authors

  • Mohammed Al Mohaini, Arshad Farid, Abdulkhaliq J. Alsalman, Maitham A. Al Hawaj, Yousef N. Alhashem, Shakira Ghazanfar, Muhammad Muzammal, Muhammad Hashim Khan, Arezoo Dadrasnia, Salmah Ismail

DOI:

https://doi.org/10.53350/pjmhs221621100

Keywords:

Streptococcus equi; breast cancer; cell proliferation; apoptosis ass

Abstract

Objective: Streptococcus equi has been identified to contain the hyaluronan synthase gene (HAS), which could have a role in the cell proliferation activity. The objective of this research was to determine anticancer properties of HAS113 recombinant clone.

Methodology: The intracellular and extracellular portions of the expressed gene products of pQE-HAS113 were harvested and evaluate for anticancer properties against prostate cancer cell (PC3) and breast cancer cell (MCF-7). To evaluate anti-proliferative properties of pQE-HAS113, two assays were carried out: MTT assay, which was used for assessing percent cell inhibition and apoptosis assay, through the AO/EB stain of living, apoptotic, as well as necrotic cells. For evaluation of the immunomodulatory properties of pQE-HAS113 on RAW264.7 in terms of IL-6, IFN-γ & IL-8 expression, flow cytometry examination was done. The harvested intracellular and extracellular portions of pQE-HAS113 were tested.

Results: The results indicated that the percentage proliferation of both the prostate cancer and breast cancer cells were 2.1% and 7.2% respectively against the intracellular portions of pQE-HAS113. While in case of the extracellular portions of pQE-HAS113 the proliferation rate of both the prostate cancer and breast cancer cells were 6.5% and 6.7% respectively. The PQE-HAS113 intracellular clone gene has shown proinflammatory activity by stimulating the expression of IL-6, INF-γ, & IL-8 in the RAW264.7 macrophage cells.

Conclusion: Thus, the results from the present study indicated that the expressed product of recombinant clone of HAS 113, pQE-HAS113 has potential to be used as anti-proliferative and apoptosis inducing substance for prostate and breast cancer.

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How to Cite

Mohammed Al Mohaini, Arshad Farid, Abdulkhaliq J. Alsalman, Maitham A. Al Hawaj, Yousef N. Alhashem, Shakira Ghazanfar, Muhammad Muzammal, Muhammad Hashim Khan, Arezoo Dadrasnia, Salmah Ismail. (2022). Screening of Anticancer and Immunomodulatory Properties of Recombinant pQE-HAS113 Clone Derived from Streptococcus Equi. Pakistan Journal of Medical & Health Sciences, 16(02), 1100. https://doi.org/10.53350/pjmhs221621100