Upregulation of Proinflammatory Variables of Analytical Importance and their Potential Interplay in the Pathogenesis of Rheumatoid Arthritis

Authors

  • Khalida Anwar, Anoosh Qayyum, Amina Shahid, Nusrat Tariq, Sultan Ahmad, Naveed Shuja, Sara Zahid, Sulayman Waquar, Arif Malik

DOI:

https://doi.org/10.53350/pjmhs22169801

Abstract

Objective: Study role of the selected inflammatory markers and their importance in the development and progression of rheumatoid arthritis.

Study Design: Cross-sectional study

Place and Duration: Institute of Molecular Biology and Biotechnology, The University of Lahore, (11-months)

Methodology: A total of 50 patients diagnosed with rheumatoid arthritis in the Department of Orthopedics (Allama Iqbal Medical College, Jinnah Hospital, Lahore-Pakistan) and 50 healthy individuals of the age of 35-45 were included into the study. Glutathione, Catalase, Maliondialdehyde, Superoxide dismutase, Glutathione peroxidase, Nitric oxide (NO) and vitamin C, E and A was estimated spectrophotometrically. Serum C-Reactive Protein, IL-21, TNF-α, AOPPs, AGE’s and MMP-3 was measured by ELISA method using Human-ELISA kits (Glory)

Results: A statistically noteworthy raise of all the biomarkers level in serum of rheumatoid arthritis (RA) was seen (p=0.001) comparable with healthy individuals. The correlation between joint damage progression diagnostic biomarker AGE and MMP-3 levels (r=0.485, 95% p=0.001) was highly significant. CRP and IL-21 also showed highly significant raise in joint inflammation (r=0.359, p=0.001). The results significantly depicted that model of MMP-3 levels at the onset of the disease and CRP and IL-21 were the robust interpreters of the erosion development. Similarly, antioxidants including Vitamin E, Vitamin C and Vitamin A were decreased in levels of (p<0.011), (p<0.011) and (p<0.022) respectively in RA subjects than in control subjects. Vitamin E, C and A are the most imperative free radical foragers within membrane. They also act as the major line of resistance against free radicals.

Conclusion: Development of pain in joints in rheumatoid arthritis condition is due to the increase in the oxidative stress which is the key contrivance in the pathogenesis of RA.

Keyword: Reactive oxygen species, Oxidative stress, oxidation of lipid peroxides, Rheumatoid arthritis, Osteoarthritis, osteocyte damage.

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How to Cite

Khalida Anwar, Anoosh Qayyum, Amina Shahid, Nusrat Tariq, Sultan Ahmad, Naveed Shuja, Sara Zahid, Sulayman Waquar, Arif Malik. (2022). Upregulation of Proinflammatory Variables of Analytical Importance and their Potential Interplay in the Pathogenesis of Rheumatoid Arthritis. Pakistan Journal of Medical & Health Sciences, 16(09), 801. https://doi.org/10.53350/pjmhs22169801