Identification of Antibacterial Efficacy of Cb6 (E)-1-(4-fluorophenyl)-3-Phenylprop-2-en-1-one against Escherichia Coli

Authors

  • Nimra Arshad, Haris Abdul Rehman, Hafiz Khawar, Muhammad Umar, Rabia Khurshid, Misbah Faiz, Aqsa Qurban, Kanza Batool, Hafiza Rumesa Yaqoob, Dania Azeem

DOI:

https://doi.org/10.53350/pjmhs22169764

Abstract

Objective of the study: Escherichia coli bacteria with increased antibiotic resistance represent a serious threat to public health. The aim of this study was to assess Cb6 (E)-1-& effectiveness against microorganisms (4-fluorophenyl), determine the effectiveness of Cb6 (E)-1-(4-fluorophenyl)-3-phenyl prop-2-en-1-one against anti-fungal agents and -3 -phenylprop-2-en-1-one against E. coli (Curvularia).

StudyDesign:Study was designed by sample collection, culturing, biochemical tests, sensitivity assay and qRT-PCR.

Place and Duration: The prospective study was carried out at the University of Agriculture in Faisalabad, Pakistan from 1st February 2019 to 18th June 2019.

Methodology: Through horizontal and vertical gene transfer, Escherichia coli acquired resistance genes and developed resistance against many antibiotic classes. The following genes were examined in the current study in E. coli: Van B, ermC, Iva-A, Qnr-A, Qnr-B, tet M, tet O, and tet W resistance genes. To assess the effectiveness of the antibacterial and antifungal agents, antibiotic sensitivity testing was carried out.

Results: E.coli chalcone resistance genes, have displayed highly significant (P 0.0001) expression in the patient sample when compared to the control.

Conclusion: It was concluded that the chalcones are highly resistant to E coli in prediction. Van B, erm C, Iva-A, Qnr-A, Qnr-B, tet M, tet O, and tet Ware resistant genes of the Escherichia coli against the Cb6 (E)-1-(4-fluorophenyl)-3 -phenyl prop-2-en-1-one and it was showed the high expression level.

Keywords: Chalcones Resistant gene, Antibacterial action, Antibiotic sensitivity, Expression.

Downloads

How to Cite

Nimra Arshad, Haris Abdul Rehman, Hafiz Khawar, Muhammad Umar, Rabia Khurshid, Misbah Faiz, Aqsa Qurban, Kanza Batool, Hafiza Rumesa Yaqoob, Dania Azeem. (2022). Identification of Antibacterial Efficacy of Cb6 (E)-1-(4-fluorophenyl)-3-Phenylprop-2-en-1-one against Escherichia Coli. Pakistan Journal of Medical & Health Sciences, 16(09), 764. https://doi.org/10.53350/pjmhs22169764