Abstract

Objective: To evaluate the effect of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in liver tissue in adult mice, orally as a supplementary agent for isoniazid-induced hepatotoxicity. Study design: Experimental study Place & Duration of study: Department of Pharmacology, Resource Lab and Department of Morbid Anatomy & Histopathology, University of Health Sciences, Lahore, Pakistan from 5th July 2019 to 5th January 2020.

Methodology: Adult male mice were divided into five groups with 7 mice in each group. Liver injury by isoniazid and its protection with oxyresveratrol was assessed by examining liver macroscopically and histological examination. We used liver homogenates for the antioxidant activity analyses. Liver samples have been tested with a few modifications for different antioxidant studies.

Results: Oxyresveratrol showed a non-significant elevation in serum glutathione levels [(1572±137.2 vs 1463±109.3) (1539±264.7 vs 1463±109.3)] respectively. However, combination therapy showed a significant increase in serum glutathione levels as compared to the INH group (1734±219.2 vs 1463±109.3). Oxyresveratrol treatment showed a significant raised serum SOD levels as rivalled to INH group (10.7 ± 0.53 vs 9.98±0.65). Treatment with oxyresveratrol and combination therapy significantly decreased pyknosis as compared to INH group (1.0±0.0 vs 1.57±0.53) (1.0±0.0 vs 1.57±0.53) and (1.0±0.0 vs 1.57±0.53) respectively. A substantial rise in parenchymal vessel congestion in the INH group as rivalled to control group (2.00±0.0 vs 1.0±0.0). Treatment with oxyresveratrol significantly decreased parenchymal vessels congestion (1.28±0.48 vs 2.00±0.0) as compared to the INH group. However, combination therapy generally showed a more significant decrease in the parenchymal vessel congestion (1.00±0.0 vs 2.00±0.0) as compared to the INH group.

Conclusion: We found a reduction in inflammation of the parenchyma and portal tract area, vascular congestion, and pyknosis may account for the anti-inflammatory activity of oxyresveratrol also, combination therapy alone significantly increased the GPx levels which confirmed their synergistic effects.

Keywords: Oxyresveratrol, Glutathione, Superoxide dismutase, Isoniazid (INH), Hepatotoxicity, LFT’s, Hepatoprotection