Abstract

Background: Staphylococcus aureus is a crucial pathogen as it can causes most common infection from skin diseases to life threatening systemic diseases. Methicillin resistant was first reported in 1961 and in the last several decades methicillin resistant Staphylococcus aureus (MRSA) is a emerging pathogen in hospitals as well as in community and most common organism isolated from different clinical samples.

Aim: To determine the frequency of methicilline resistant Staphylococcus aureus from different clinical samples in specific area of Karachi Gadap Town.

Study design: Descriptive case series

Place and duration: Department of Medical Technology, Baqai Medical University Karachi 1st January 2015 to 30th June May 2015

Methodology: A total of 100 clinical samples of pus were collected from Laboratory of Fatima Hospital Baqai Medical University Karachi. Clinical samples were isolated and cultured Staphylococcus aureus was identified using standard microbiological methods recommended of clinical laboratory standard institute (CLSI) methicillin resistance was confirmed using cefoxitin and oxacillin disks.

Results: Sixty two were Staphylococcus aureus, 27 isolates shows methicillin resistance so the frequency of MRSA 43.5%.

Conclusion: Staphylococcus aureus dominating organism among pus samples and high rate of methicillin resistance which is alarming, in particular, concern on the rational use of antibiotics.

Keywords: Frequency, Methicillin resistant Staphylococcus aureus, Surgical wound