Abstract

Background: Endothelin is an inflammatory marker secreted from endothelium and disturbed in inflammatory condition.

Aim: To evaluate the effects of endothelin in polycystic ovarian syndrome (PCOs).

Methods: This study was comprised of two groups of women, group A, 48 women with polycystic ovarian syndrome using Rotterdam criteria and group B was composed of 48 women of reproductive age having no PCOS. Detailed information about age, body mass index (BMI), duration of menstrual cycle, marital status and clinical features like obesity, infertility, acne and hairs on face were noted. A total 5ml venous blood was collected by using aseptic techniques and then centrifuged immediately; serum was separated and stored at -20°C after proper labeling. Samples were analyzed for estimation of serum endothelin by using enzyme linked immunosorbent assay kit (ELISA). Statistical analysis was done by using statistical package for social sciences (SPSS) 21. The p value <0.05 was considered to be statistically significant.

Result: After performing ELISA test of endothelin, it was revealed that mean±SD of endothelin for PCOS women was 5.2±1.05 and mean±SD of healthy women was 3.65±0.51 and median (IQR) of endothelin in PCOS women was 4.9 (1.28) and median (IQR) of healthy women was 3.65 (0.85) and p value of endothelin, generated by Mann- Whitney U test was < 0.001.

Conclusion: It is concluded that the level of endothelin is increased in PCOS as compared to healthy women in which the level of endothelin is decreased or within normal ranges.

Keywords: Endothelin, Inflammation, Polycystic ovarian syndrome, Enzyme linked immunoassay absorbant,