Abstract

Objectives: To identify the isolates by means of different identification tests, to assess the frequency of Klebsiella pneumoniae, biofilm formation ability and antibiotic susceptibility of biofilm forming isolates.

Study Design: Cross-sectional/Observational study

Place and Duration: Conducted at Microbiology Laboratory, Gujranwala Medical College / D.H.Q Teaching Hospital Gujranwala Pathology Lab from August 2020 to October 2020.

Methodology: A total of 250 urine catheter tips samples were collected from patients admitted in ICU and CCU. This study includes the methodology in which the study organism was inoculated and confirmed by standard microbiological methods including culture inoculation, staining, biochemical tests, biofilm formation tests and antibiotic sensitivity. Qualitative method has been used to see biofilm formation in Klebsiella pneumoniae isolates. 10 different CLSI recommended necessary disc of antibiotics were used to observe the sensitivity and resistance of Klebsiella pneumoniae by Kirby-Bauer disc diffusion method.

Results: There were 170 (68%) males and 80 (32%) females. Majority of patients 160 (64%) were ages 46 to 66 years. 75 samples were confirmed by morphological confirmation, biochemical tests and microscopic analysis with those of Klebsiella pneumoniae. From 75 confirmed isolates of Klebsiella pneumoniae, 17 shows strong biofilm formation, 33 shows weak biofilm formation and 25 shows no biofilm formation. Total number of biofilm forming Klebsiella pneumoniae are 50 out of 75 confirmed isolates which are 66.66% respectively. Out of 50 biofilm forming Klebsiella pneumoniae isolates 41 shows multi-drug resistance (MDR) which are 82% respectively. Out of 10 drugs that were used Amikacin, Tazocin and Sulzone were found to be most sensitive against Klebsiella pneumoniae while Augmentin and Ciprofloxacin were found to be most resistant against Klebsiella pneumoniae.

Conclusion: This study shows that higher percentage of extracted organism shows biofilm formation and from these biofilm forming isolates maximum shows multi-drug resistance. Further advances in the prevention of nosocomial infections will require new approaches to control infections.

Keywords: Urinary catheter tips, Klebsiella pneumoniae, Biofilm formation, Antibiotic susceptibility, Multi-drug resistance.