Abstract

Background: The rising resistance of Pseudomonas aeruginosa, E. coli, Klebisella, Streptococcus and Entrococcus many antimicrobials poses a major therapeutic challenge; Carbapenen-resistant Pseudomonas aeruginosa, in particular, exhibit variation in resistance patterns having a great concern in hospitals due to its nosocomial infections.

Aim: To identify the Prevalence of Pseudomonas aeruginosa, to compare the outcomes for patients infected with Pseudomonas aeruginosa and to look for the antimicrobial activity of antibiotics against Pseudomonas aeruginosa. Study design: Experimental study Place and duration of study: Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore from 1st March 2017 to 28th February 2018. Methodology: Fifty urine samples of pregnant women with age range was from 22-45 were studied. Initially, the isolates were identified by colony morphology and different biochemical tests and then by applying Analytical Profile Index (API). Consecutive Pseudomonas aeruginosa cultured from different specimens were challenged with locally available antibiotics using the standard disc diffusion method. Results: The mean age of the women was 46.1 years. There were 18 positive and 32 were no growth. However E. coli was seen only in 7 specimens, Klebisella in 3, Pesudomonoas in 2, Streptococcus in 5 and Entrococcus in 1 specimen.

Conclusion: The prevalence of Pseudomonas aeruginosa, E. coli, Klebisella, Pesudomonoas, Streptococcus and Entrococcus were 5% revealed.

Key words: Pseudomonas aeruginosa, Nosocomial pathogen, Carbapenem-resistant, E. coli, Klebisella.