Abstract

Aim: To evaluate anticancer potential of zinc oxide nanoparticles individually and in combination with various conventional antibiotics against different multidrug-resistant bacterial isolates and validation of clinical parameters for measuring treatment efficacy in rat organs (liver) through biopsy.

Study design: Experimental study

Place and duration of study: Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore from 1st July 2019 to 30th June 2020.

Methodology: Zinc oxide nanoparticles with the purity of 99.5% obtained from IMBB, used for preparing Stock suspension (1 mg mL-1) freshly in phosphate-buffered saline (PBS, pH 7.4) and sonicated prior to exposure. Male rats purchased from PGMI, Lahore were housed in animal cages at animal laboratory in ventilated room maintained at 25 °C±2°C and 70%±10% relative humidity with 12-hour light/dark cycle.

Results: No tumor in the liver was found and best results were obtained in liver function test. Liver disorder which cannot be reversed known as Liver cirrhosis was also observed. This disorder was observed with regular scarring of tissues of liver along with nonstop regeneration. Some of the major factors like alcohol and viral infections, etc. are among major reason for Liver cirrhosis.

Conclusion: Zinc oxide nanoparticles are the nanoparticles which have ability to induce apoptosis as well as release of reactive oxygen species (ROS) in cells. Hence zinc oxide nanoparticles are considered as a great nanoparticle against cancer and microbes.

Keywords: Cancer, Zinc oxide nanoparticles (ZnO-NPs), Chemotherapy, Radiotherapy, Albino rats