Nurses got exposed to various blood borne infections due to injury from different types of sharp instruments like needles, lancets, scissors etc. Such injuries ultimately became the reasons of blood borne diseases. Objectives: To analyze the knowledge and attitude of nurses about prevention from needle stick injury and the magnitude of such incidents in Tertiary Care Hospitals of Lahore. Study Design: Cross-sectional study. Methodology: Study setting was tertiary care hospitals of Lahore-Pakistan after ethical review committee’s (ERC) approval. Data was collected from nurses by using self-administered questionnaire from different department of tertiary care hospitals, Lahore. Convenient sampling technique was used, and sample size was 93. Statistical analysis: SPSS 21 was utilized for the data analysis. The tests of frequencies, percentages and graphs were applied. Results: Needle stick injuries association was measured with working experience among nurses. There was statistically significant difference found between the NSIs among (59%) nurses, who were having more working experience (22-24) about handling of needle during any procedure as compared to the nurses who lack in experience. The study results showed that majority of nurses have enough knowledge concerning to NSIs. Conclusion: We concluded that nurses have knowledge regarding the needle stick injury due to the training classes, however, ignorance of the protocols becomes the reason of such incidents even now. It is the need of time to implement appropriate strategies for precaution and intervention through hospital administration to avoid the needle stick injuries. Key Words: Needle Stick Injury, Re-capping of needles, Attitude and Knowledge