ABSTRACT

This study was aimed at assessing the types of bacterial isolates and their antimicrobial agentsprofile identified from raw meat and meat products butcher shops indifferent areas of Karbala city,A total of 100 random samples from raw meat were purchased from different butcher shops in Karbala city between October 2019 to February 2020 for the isolation and identification of microbial pathogens, The antibiotic resistant characteristics of the isolated organisms was determined by using Kirby-Bauer disk diffusion method and Polymerase chain reaction assaywas used to confirmall bacterial isolates obtained from direct colony of 24 hour by using16S rRNA genes, the results showed Escherichia coli was most Gram-negative found on meat samples about(45 samples) with percentage 45% fromall samples, 30% from samples contaminated with Gram-positivebacteria (Staphylococcus) and 6% was recorded as Salmonella, on the other hand , The results showed that the Ciprofloxacin and Gentamicin were susceptible to 95% of all isolates.

Keywords: Staphylococcus, Salmonella, E.coli, PCR, raw meats