ABSTRACT

Background: Heavy metals and their chemical salts are source of great threat to human and animals alike.

Aim: To observe hepato-protection by vitamin-E on sodium arsenite induced histomorphological changes of liver on albino rats.

Study Design: Cross Sectional study.

Methodology: 90 adult albino male rats (30 in each group), weighing 140 to 180 grams were taken. They were housed in separate stainless steel cages and were kept under controlled temperature of 23+2 and humidity 50+5% in the experimental research lab of postgraduate medical institute Lahore. The night and day cycle was maintained for 12 hours each. Each animal of every group was labeled from 1-30 with different color for every group. Standard diet and tap water ad libitum was given to animals. Before starting the experiment animals were acclimatized for 10 days. Data analyzed by SPSS 22.0v.

Results: The initial and final body weights of animals in all the three study groups were shown in table-1 as mean ± SD. The relative tissue weight index (RTWI) of liver was measured and group wise comparisons were made for RTWI was found significant with p-value <0.001. The difference for diameter of hepatocyte among three groups was significant with p-value <.001.

Conclusion: This study concluded that there is clear evidence of improvement with vitamin E hence it can be safely stated that vitamin E provides hepatoprotection against arsenic induced hepatotoxicity.

Keywords: Vitamin-E, Hepato-protection, Relative Tissue Weight Index and Albino Rats.