Aim: To find out the frequency of vitamin D and calcium in pregnant women and their effects on fetus as well as mothers. Study Design: Retrospective study. Place and Duration of Study: Darul Shifa Clinic & Maternity Home Karachi Pakistan from 1st July 2020 to 31st December 2020. Methodology: One hundred and twenty patients with proper evidence of pregnancy were enrolled. All patients from age 18 years to age 35 years with no other disease were included and those women who have some other diseases or age outliers were excluded. Results: There is a significance difference in the vitamin D and calcium during the period of pregnancy. In the group A (18 years to 25 years) the significance difference of vitamin D was (P=0.000) while the calcium was (P=0.200). In the group B (26 years to 30 years) the significance difference of vitamin D was (P=0.000) while the Calcium was (P=0.071) and in the group C (30 years to 35 years) there was no significant change of vitamin D and calcium (P=0.000). The result of group C is different from the results of the above two groups, because in this age majority of the women were living in a congested houses as well as were spending a depressive life due to home problems, husband wife compromise problems, children problems, financial problems etc. and all such problems have a direct impact on their pregnancy Conclusion: It was statistically found that the Vitamin D and calcium values show significances changes during pregnancy in contrast to the normal women. Key words: Vitamin D, Calcium, Pregnant women