Background: Diabetic neuropathy is common in diabetes mellitus patients with the prevalence of 50%. Inflammation takes a vital role in the complex pathogenesis of diabetic neuropathy. The decreased inflammation status is becoming a potential target of therapy and prevention of diabetes complication.

Objective: to investigate the supplementation effect of vitamin B6, folic acid, and vitamin B12 in combination to inflammatory status of diabetic neuropathy patients.

Method: experimental design with randomized controlled trial. Subject of study diabetic neuropathy patients. Place of study Tugurejo Hospital, Semarang in September 2018 to March 2019, with sample size of 75 subjects (treatment and control group).

Results: Subjects of the study consists of 56 females and 19 males. The characteristic of subject in treatment and control group is not significantly different based on sex, vitamin B6, folic acid, vitamin B12 intake, and nutritional status. Compared with placebo, the effect of vitamin B6, folic acid, vitamin B12 co-supplementation is able to decrease serum Hs-CRP significantly with mean difference of -3.0 (CI 95% -4,95, -1,08; p <0,05.

Conclusion: Co-supplementation of vitamin B6, folic acid, vitamin B12 is able to significantly decrease inflamation status.

Keyword: Folic acid, vitamin B6, vitamin B12, inflammation status, diabetic neuropathy