This study describes anti-hyperglycemic effect of Momordicacharantia extract commonly known as bitter melon. Random blood glucose levels were observed before and after plant extract administration. Powdered form of plant extract was used as an oral treatment. Diabetes was induced in animal (mice) models by using streptozotcin which is an artificial diabetes inducer. Maximum anti-hyperglycemic effect and blood glucose level reduction was observed in individual treatment of Momordicacharantiaextract (500mg/kg) which was 75% ± 1.3. This treatment was more effective as compared to Amaryl (3mg/kg) which shows effectiveness of 52% ± 2.4 and Glucophage (500mg/kg) which shows effectiveness of 29% ± 2.1. Results indicate that bitter melon contain anti-hyperglycemic proteins which are helpful in diabetes treatment without any toxic side effects. Still more research, experiments and testing needs to be perform. Keywords: Amaryl; Anti-hyperglycemic effect; Blood glucose levels; Body weight; Diabetes mellitus