Inflection of Oxidative Status of Nepthalene Induced Liver Injury in Mice
Iram Arshad, Anam Arshad, Muhammad Humayun, Arif Malik
701
ABSTRACT
Background: Cancer is a leading cause of morbidity and mortality worldwide.
Numbers of therapeutic strategies are being used to target aberrant pathways
involved in malignant cell proliferation and survival. The increasing side
effects and evolution of resistant population has driven scientists to device
newer and targeted therapeutic options to be used.
Aim: To investigate the hepatotoxic effect of intraperitoneal administration
of naphthalene derivatives in mice.
Methods: Naphthalene derivative (Naphthoquinone) was dissolved in DMSO (Dimethyl
Sulfoxide) and given to the white albino mice to compare it with control group
after 30 days of administration. The fifteen mice used for the experiment were
divided into 3 groups (A, B, C). Group B and C received 50 mg/kg and 150 mg/kg
per body weight intraperitoneally respectively, whereas control group (A)
received no drug. Biochemical assessment of ALT, AST, ALP, Albumin, Cholesterol
and triglycerides were done by kit method, histological analysis was done and
stress markers (MDA, GSH, SOD, and Catalase) were analyzed using
spectrophotometer.
Results: Naphthalene derivative toxicity was observed as the result showed that
mean serum ALT, AST and ALP level were higher in mice administered naphthalene
derivative as compared to control group. In addition, histological changes such
as inflammation leading to cirrhosis were seen in liver on different doses,
other biomarkers of toxic effects include glutathione depletion, lipid
peroxidation, DNA fragmentation and the production of reactive oxygen species
(ROS).
Conclusion: We concluded that naphthalene derivatives metabolism triggers
production of ROS, coupled with impaired oxidant/antioxidant balance, leading
to a state of oxidative-stress that could have been partially responsible for
the slight hepatotoxicity and the disturbance in the level of hepatic enzymes
seen in this study. Therefore, a possible conclusion that such biochemical
changes observed in these experimental animals may be seen in human beings is
undeniable so naphthalene derivatives may have more toxic effects than
therapeutic effects.
Keywords: Hepatotoxicity,
Oxidative Stress, Antioxidants, Naphthalene, Naphthoquinone, DMSO
ABSTRACT
Background: Cancer is a leading cause of morbidity and mortality worldwide.
Numbers of therapeutic strategies are being used to target aberrant pathways
involved in malignant cell proliferation and survival. The increasing side
effects and evolution of resistant population has driven scientists to device
newer and targeted therapeutic options to be used.
Aim: To investigate the hepatotoxic effect of intraperitoneal administration
of naphthalene derivatives in mice.
Methods: Naphthalene derivative (Naphthoquinone) was dissolved in DMSO (Dimethyl
Sulfoxide) and given to the white albino mice to compare it with control group
after 30 days of administration. The fifteen mice used for the experiment were
divided into 3 groups (A, B, C). Group B and C received 50 mg/kg and 150 mg/kg
per body weight intraperitoneally respectively, whereas control group (A)
received no drug. Biochemical assessment of ALT, AST, ALP, Albumin, Cholesterol
and triglycerides were done by kit method, histological analysis was done and
stress markers (MDA, GSH, SOD, and Catalase) were analyzed using
spectrophotometer.
Results: Naphthalene derivative toxicity was observed as the result showed that
mean serum ALT, AST and ALP level were higher in mice administered naphthalene
derivative as compared to control group. In addition, histological changes such
as inflammation leading to cirrhosis were seen in liver on different doses,
other biomarkers of toxic effects include glutathione depletion, lipid
peroxidation, DNA fragmentation and the production of reactive oxygen species
(ROS).
Conclusion: We concluded that naphthalene derivatives metabolism triggers
production of ROS, coupled with impaired oxidant/antioxidant balance, leading
to a state of oxidative-stress that could have been partially responsible for
the slight hepatotoxicity and the disturbance in the level of hepatic enzymes
seen in this study. Therefore, a possible conclusion that such biochemical
changes observed in these experimental animals may be seen in human beings is
undeniable so naphthalene derivatives may have more toxic effects than
therapeutic effects.
Keywords: Hepatotoxicity,
Oxidative Stress, Antioxidants, Naphthalene, Naphthoquinone, DMSO