In the current scenario Multi Drug Resistant (MDR) bacterial pathogens are  increasing very rapidly due to the misuse of antibiotics to control antibiotic resistance, we need to know the current pattern of antibiotic resistance. Furthermore, new bioactive metabolites are needed for the alternative option to control antibiotic resistance. Fungi have the ability to produce potent metabolites. The production of secondary metabolites such as antimicrobial agents is the most important property of fungi. Antimicrobial activity of extract were noted against different pathogenic (Clinical Isolates) and using agar well diffusion assay method and noted different zone of inhibition of each bacteria, wells were loaded with different concentration’s 50 µL and 100 µL. Maximum zone of inhibition were reported at different concentration of different bacteria. Ethyl acetate crude extract at 50 µL concentration’s 17 ± 0.15 mm, 18 ± 0.1 mm , 16 ± 0.4 mm, 19 ± 0.26 mm, 17 ± 0.30 mm,16 ± 0.36 mm, zone of inhibition against E.coli sp, Pseudomonas, Klebsiella sp Proteus sp, S,aureus, , and Salmonella sp, respectively. While in 100 µL zone of inhibition was noted,  21 ± 0.15 mm, 22 ± 0.40 mm, 20 ± 0.35 mm, 21 ± 0.26 mm, 22 ± 0.45 mm 19 ± 1.10 mm zone of inhibition against E.coli sp, Pseudomonas, Klebsiella sp Proteus sp, S,aureus, , and Salmonella sp, respectively. In the current study we used FTIR and Plasmon Resonance (SPR) UV-visible spectroscopy observed at 408 nm during UV-visible spectroscopy. FTIR analysis revealed the involvement of phenolic, carboxyl and hydroxyl groups in reduction of Ag⁺ ions to form fungal metabolites while stabilization components of were fungal metabolites amide linkage and amino acid.

Keywords; Aspergillus fumigatus, Secondary metabolites, Ethyl acetate, FTIR, UV-visible spectroscopy, MDR pathogen.