Objective: To assess the autopsy in relation with age and gender in tertiary care hospital of Hyderabad, Sindh, Pakistan.

Study Design: Retrospective, observational and non-interventional study

Place and Duration of Study: Medicolegal Department of Liaquat University of Medical and Health Sciences Hyderabad from 1st January 2017 to 31st December 2018.

Methodology: Three hundred and eighty-one patients were enrolled. Results: According to cause of death, 88 (23.10%) were died due to road traffic accident, firearm injury 73 (19.16%) and asphaxial death 70 (18.37%), assault 65 (17.06%), poisoning 37 (9.71%), electric shock 30 (7.87%) and undetermined 18 (4.72%) respectively. Conclusion: The relatable factors such as age and gender with the suicidal cases in results of autopsy examination which plays the most relevant role in the medical practices even after the advancement of diagnostic technologies. Key words: Assess, Autopsy, Age, Gender, Tertiary care hospital