Kappa Test With Platelet Rich Plasma (PRP) and Platelet Poor Plasma (PPP) Blood Preparation Method for Examining the Value of Activated Partial Tromboplastin Time (APTT) and Plasma Protrombin Time (PPT)
Ratih Hardisari, Furaidakhasanah, Budi Setiawan, Suyana
1397
ABSTRACT
Examining
the Activated Partial Thromboplastin (APTT) and Plasma Protrombin Time (PPT) is
sort series of examining homeostasis which is conducted in order to have
screening test for homeostasis disorder. This examination used plasma sample in
which solidification factors were found which could be influenced by
thrombocyte existence. The centrifuging of citrate blood sample which was
conducted too fast or too slow would cause plasma conditions with the number of
thrombocytes. Practical in some laboratories were not yet been uniformed,
mainly in centrifuging of citrate blood to obtain citrate plasma with fewer
thrombocyte contents. To identify the value of Kappa, it is by compatibility
test between two methods PRP and PPP to examine PPT and APTT. This is true
experimentalstudy with post-test research design without control. The citrate
plasma sample was obtained from 10 samples of students’ blood which had one
pair, 2 treatments; by centrifuging 3000 rpm for 10 minutes for PPP and
centrifuging 1000 rpm for 10 minutes for PRP. Then, both methods (PPP and PRP)
were examined by using PPT and APTT parameter. In the result of PPT examination
in the sample of PRP plasma, the average value was 11.6 seconds. In the sample of PPP, the average value was
11.0 seconds. The result of APTT examination in PRP sample, the average value
was 34.27 seconds while in PPP sample was 33.18 seconds. There was
compatibility in the result, either PPP method and PRP for PPT and APTT
examination (Kappa = 1).
Keywords: PRP, PPP, APTT, PPT, Kappa
ABSTRACT
Examining
the Activated Partial Thromboplastin (APTT) and Plasma Protrombin Time (PPT) is
sort series of examining homeostasis which is conducted in order to have
screening test for homeostasis disorder. This examination used plasma sample in
which solidification factors were found which could be influenced by
thrombocyte existence. The centrifuging of citrate blood sample which was
conducted too fast or too slow would cause plasma conditions with the number of
thrombocytes. Practical in some laboratories were not yet been uniformed,
mainly in centrifuging of citrate blood to obtain citrate plasma with fewer
thrombocyte contents. To identify the value of Kappa, it is by compatibility
test between two methods PRP and PPP to examine PPT and APTT. This is true
experimentalstudy with post-test research design without control. The citrate
plasma sample was obtained from 10 samples of students’ blood which had one
pair, 2 treatments; by centrifuging 3000 rpm for 10 minutes for PPP and
centrifuging 1000 rpm for 10 minutes for PRP. Then, both methods (PPP and PRP)
were examined by using PPT and APTT parameter. In the result of PPT examination
in the sample of PRP plasma, the average value was 11.6 seconds. In the sample of PPP, the average value was
11.0 seconds. The result of APTT examination in PRP sample, the average value
was 34.27 seconds while in PPP sample was 33.18 seconds. There was
compatibility in the result, either PPP method and PRP for PPT and APTT
examination (Kappa = 1).