ABSTRACT

Background: There were conventional reportes about overexpressionrate HER2)Human epidermal growth factor receptor) in breast cancer.

Aim: To determine HER2 gene over expression using immunohistochemical method in Breast cancer.

Methods: This descriptive analytical research was conducted on 74 breast cancer samples admitted in hospitals in Isfahan Iran. Each sample was fixed in formalin and tissue processing and sectioning were performed . The slides were stained by H/E. All samples were diagnosed by two expert pathologists. Determination of HER2gene expression was done after antigen retrieval with primary specific rabbit monoclonal HER2antibody by immunohistochemistry. Data was analyzed with SPSS16 using with chi- squre tests.

Results: Out of 74 specimens of breast cancer 39(52.7 % ) specimens detected of HER2gene over expression but 35(47.3%) samples malignancy were not showed HER2 gene expression. There was significant (P<0.026)relationships between high stage tumor with over expression of HER2 gene. Among 39 specimens HER2 positive 6 samples (8.1% ) were in grade one, 18 samples (24.3%) were in grade two and 15(20.3%) samples had grade three. There was significant (p=0.029). relationships between HER2 gene expression with grade tumor

Conclusion: This study showed that patients HER2 who were over 50 years old had HER2positive more those under fifty. Patients in high stage had more HER2 over expression and poor predictive value compared to low stage .

Keywords: Breast cancer, Immunohistochemistry,HER2