Effects of cisplatin on renal biochemical and histological picture in male albino rats Role of propolis
Maher Ibrahim Ismael, Imad Abdul-Jabbar Thanoon, Mohammad K. Shindala
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ABSTRACT
Aims: This study aimed to investigate the effects of cisplatin renal function
tests with histological picture, and any
possible role for propolis in ameliorating toxic changes, in comparison to
controls.
Materials and Methods: This experimental work takes 6 weeks and involved 60 male albino rats
,weighed 230±40g , divided into 6
groups, each of 10 members.
Group A, received 3 doses of cisplatin at 4 mg/kg intraperitoneally (I.P) at
weekly intervals starting by the end of the 2nd week, and killed by
the end of the 6th week.
Group B, received propolis at 120 mg
/day orally for 6 weeks.
Group C, received propolis 120 mg/day initially then by the end of the 2nd
week started cisplatin 4mg I.P weekly for 3 doses.
Group D, received propolis 60 mg/day for 6 weeks.
Group E, received propolis 60 mg daily for 6 weeks, starting cisplatin I.P 4 mg
3 doses by the end of the 2nd week ,one week apart.
Group F, represent the control group, on normal saline orally for 6 weeks.
At the start the animals
in all groups were weighed ,a blood
sample was taken then, during the experiment , weighed , physical activity and
food intake were recorded, by the end of the experimental duration weight were
recorded, a blood sample were taken and the animals were killed , kidneys were taken for their weight and histological
section with H& E staining.
The blood samples taken
before and after the experiment were blood urea, serum creatinine, serum uric
acid.
Results: By observation: By comparison of before and 6 weeks after the experiment
, there was a significant weight reduction in the group A, with loss of 2
members of this group after the 3rd dose of cisplatin. While group B
showed a significant weight gain. Clear and significant changes were recorded
in parameters of renal function tests , in group A in comparison to control
group, with a clear ameliorative effects of propolis especially noticed at 120
mg/d.
Also there was a clear
changes in the histological picture of the kidney with obvious ameliorated
effects of propolis especially noticed
at 120 mg dose /day.