Hepatotoxic Potential of Doxorubicin in Albino Rats
Amber Shami, Muhammad Bahadur Baloch, Attiya Mubarik Khalid, Fouzia Farzana, Maryam Nasim, Muhammad Muneeb Ather
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ABSTRACT
Background: Doxorubicin is an effective chemotherapeutic drug, but its toxic
potential limits its use.
Aim: To study the effects of doxorubicin on the relative tissue weight index
and hepatic central vein diameter in the albino rats.
Study
Design: Experimental study
Place and
Duration of study: Study was conducted on 20 adult albino rats
of both male and female gender in the animal house of Anatomy department,
(PGMI) Lahore, Pakistan in April 2019.
Materials
& Methods: Twenty, healthy adult albino rats of either
sex, aging 6 to 8 weeks with 180-220 g of weight were randomly allotted to two
groups A and B, each group having ten animals. Standard Rat feed and distilled
water were given intraperitonially in the dose of 1.2mg/kg/body weight to the
control group whereas doxorubicin was given intraperitoneally in the dose of
1.2mg/kg/body weight to the experimental group twice a week for 21 days. Body weight along with relative Tissue Weight Index were
measured in gms at the end of the experiment. Diameter of central vein (µm) was
assessed histologically.
Results: The mean weight of the liver decreased significantly in the experimental
group. On the contrary, the relative tissue weight index in all groups were
significantly different according to the One-way-ANOVA test. The increase in
the diameter of the central vein in the drug administered group commenced on
significant basis (p-value < 0.001)
Conclusion: Doxorubicin has toxic effect on the relative tissue weight index and
hepatic central vein diameter in the albino rats
KeyWords: Doxorubicin, Liver weight, relative tissue weight index, Central vein
diameter, Liver, Hepatoxicity, Albino rats
ABSTRACT
Background: Doxorubicin is an effective chemotherapeutic drug, but its toxic
potential limits its use.
Aim: To study the effects of doxorubicin on the relative tissue weight index
and hepatic central vein diameter in the albino rats.
Study
Design: Experimental study
Place and
Duration of study: Study was conducted on 20 adult albino rats
of both male and female gender in the animal house of Anatomy department,
(PGMI) Lahore, Pakistan in April 2019.
Materials
& Methods: Twenty, healthy adult albino rats of either
sex, aging 6 to 8 weeks with 180-220 g of weight were randomly allotted to two
groups A and B, each group having ten animals. Standard Rat feed and distilled
water were given intraperitonially in the dose of 1.2mg/kg/body weight to the
control group whereas doxorubicin was given intraperitoneally in the dose of
1.2mg/kg/body weight to the experimental group twice a week for 21 days. Body weight along with relative Tissue Weight Index were
measured in gms at the end of the experiment. Diameter of central vein (µm) was
assessed histologically.
Results: The mean weight of the liver decreased significantly in the experimental
group. On the contrary, the relative tissue weight index in all groups were
significantly different according to the One-way-ANOVA test. The increase in
the diameter of the central vein in the drug administered group commenced on
significant basis (p-value < 0.001)
Conclusion: Doxorubicin has toxic effect on the relative tissue weight index and
hepatic central vein diameter in the albino rats
KeyWords: Doxorubicin, Liver weight, relative tissue weight index, Central vein
diameter, Liver, Hepatoxicity, Albino rats