Background: Polycystic ovary syndrome (PCOS) influences almost 18% of women in their child bearing age and responsible for reproductive, metabolic and psychological dysfunctions.

Aim: To examine remedial effect of Nigella sativa on body weight, weight, volume and gross appearance of ovary in polycystic ovarian syndrome induced by Letrozole in mice.

Study Design: Randomized control trial.

Methodology: Mature female BALB/c mice (n=40) were placed in four groups having ten in each. Group-1 was fed with normal diet. Rests of three were treated with Letrozole at dose of 1mg/kg body weight once daily for eight weeks. Groups (3&4) were also given Nigella sativa seeds powder and oil at dose of l0g/kg body weight and 4ml/kg body weight respectively, once daily commencing at 23 day and remained at end of eight weeks. Ovaries were identified, shape and color were recorded then weighed using a digital precision balance and volume in mm3 by water displacement method.

Statistical analysis: Data analyzed by SPSS 21.0v.

Results: Body weight of group-2 animals, weight and volume of their ovaries was increased in comparison to group-1 but reduced to group-3 and group-4 animals. Blackish spots on group-2 animal ovaries were observed. Ovaries of groups (3&4) animals were normal in appearance, volume and weight

Conclusion: This study concluded that Nigella Sativa seeds in both solid and liquid state leave identical curative impact on body weight, weight, volume and gross appearance of ovaries in polycystic ovarian syndrome in mice.

Keywords: Body Weight, Letrozole, Nigella Sativa, Ovaries and Polycystic Ovarian Syndrome