ABSTRACT

Aim: To eliminate or reduce lead poisoning in experimental animals (rats) by the biological action of Lactobcillus

casei.

Methods: The purity of the isolates of this bacterium was confirmed based on the culture and microscopic characteristics and the biochemical tests. A lead acetate solution was prepared at a concentration of 2 mg / mL. A biological experiment was designed in rats with 3 treatments, these are: the treatment of infection by dosing with lead acetate solution and the treatment by dosing with a liquid bacterium Lactobcillus casei. After the end of the trial period of (11 weeks), the animals were killed and blood samples were taken for which tests were conducted for the parameters of blood images, blood lipids, creatine and urea, and liver enzymes. The animals were also dissected and extracted from them the organs of the brain, liver and kidneys, ashes were estimated for these three members and the element lead was estimated in samples of the organs ashes.

Results: The results showed that treatment with the Lactobcillus casei probiotic booster had a clear significant effect on the parameters of blood images (WBC, PCV, RBC, HB), cholesterol, creatinine, and the liver enzyme (ALP), whereas, the treatment with this bacteria returned these values to the same values in a healthy control experiment.

Conclusion: The treatment gave significant positive results without treating or reducing the concentration of lead to normal concentration (healthy control) in liver, kidney and brain samples.

Keywords: L.casei, Lead Pb, blood and lipid profile, liver enzymes