ABSTRACT

Objective: The aim of this study is to determine the etiology and antimicrobial patterns of neonatal sepsis.

Study Design: Cross-Sectional

Place & Duration: Paediatric department of Alkhidmat Hospital Peshawar for six months duration from June, 2019 to December, 2019.

Methods: Total 340 blood samples of both genders were extracted in this study. Patients demographics were recorded after written consent. All the patients were worked up with complete history, clinical examination and investigations to rule out confounders and bias in the study results. All the follow ups were done by the researcher himself. Antimicrobial susceptibility testing was carried out by Modified Kirby Bauer disk diffusion method on Mueller Hinton agar using CLSI protocols.

Results: In this study, 93 (27.35%) were culture positive extracted from 340 blood samples. Out of 93 positive blood cultures, 52 (55.91%) were observed gram negative organisms and gram positive organisms were 34 (36.56%) and the rest were positive for Candida spp 7 (7.53%).Pseudomonasspp 18(34.62%) was the most common isolated in gram negative organisms while in gram positive organisms Coagulase Negative Staphylococci (CoNS) 23 (67.65%) was the most frequent isolated organism.Variable pattern of resistance was seen among other members of enterobacteriaceae, non-fermenters and Gram positive organisms.

Conclusion:We concluded in this study that the occurrence of gram-positive isolated species was lower in number than that of gram-negative organisms due to the isolation of gram-positive coagulase-negative staphylococci (CoNS) and gram-negative Pseudomonas spp.

Keywords:Neonatal sepsis, Bacteriological profile, Antibiotic susceptibility, Neonates