ABSTRACT

Aims: This study aimed to investigate the effects of cisplatin on oxidative stress parameters (malondelhyde MDA; Total antioxidant status TAS) , liver function tests with histological picture and any possible role for propolis in ameliorating toxic changes, in comparison to controls.

Materials and Methods: This experimental work takes 6 weeks and involved 60 male albino rats ,weighed 230±40g , divided into 6 groups, each of 10 members.

Group A, received 3 doses of cisplatin at 4 mg/kg intraperitoneally (I.P) at weekly intervals starting by the end of the 2nd week, and killed by the end of the 6th week.

Group B, received propolis at 120 mg /day orally for 6 weeks.

Group C, received propolis 120 mg/day initially then by the end of the 2nd week started cisplatin 4mg I.P weekly for 3 doses.

Group D, received propolis 60 mg/day for 6 weeks.

Group E, received propolis 60 mg daily for 6 weeks, starting cisplatin I.P 4 mg/kg 3 doses by the end of the 2nd week ,one week apart.

Group F, represent the control group, on normal saline orally for 6 weeks.

At the start the animals in all groups were weighed ,a blood sample was taken then, during the experiment , weighed , physical activity and food intake were recorded, by the end of the experimental duration weight were recorded, a blood sample were taken and the animals then were killed , livers were taken for their weight and histological section with H& E staining.

The blood samples taken before and after the experiment were analysed for ALT, AST, ALP, serum albumin, total serum bilirubin, direct and indirect, serum MDA and TAS levels.

Results: By observation: By comparison of before and 6 weeks after the experiment , there was a significant weight reduction in the group A, with loss of 2 members of this group after the 3rd dose of cisplatin. While group B showed a significant weight gain. Clear and significant changes were recorded in parameters of liver function tests, MDA and TAS levels in group A in comparison to control group, with a clear ameliorative effects of propolis especially noticed at 120 mg/d. Also there was a clear changes in the histological picture of the liver with obvious ameliorated effects of propolis especially noticed at 120 mg dose /day.

Conclusion: Propolis administration at a daily dose of 120 mg/day clearly ameliorated the toxic effects of cisplatin on the liver at both functional and histological pictures