Background: Diabetes Mellitus is one of the alarming health hazardous indicator and with poor glycemic control it will be dangerous for human health because more prone to development of different systemic & vascular complications. G6PD is one of the carbohydrate metabolic enzyme which prevent from oxidative stress and development of free radicals.

Objective: The purpose of this research to evaluate the level of G6PD in patients of type-2 diabetes mellitus with their different levels of glycemic control index.

Methodology: This study was done at Department of Biochemistry LUMHS with collaboration of Diabetic clinic LUMHS and Diagnostic & Research Laboratory LUMHS Jamshoro. Total 100 diagnosed cases of type-2 diabetes were selected and divided into three groups with different categories of glycemic control index. The HbA1c% was measured by Bio Red Variant while G6PD was measured by standard G6PD quantitative measurement method at Diagnostic laboratory LUMHS using assay kit (SD Biosensor, Inc. Republic of Korea).

Results: The G6PD significantly (p < 0.05) decline in diabetic patients with poor glycemic control having mean values of HbA1c% between 11-13%.

Conclusion:  This research study concluded that there is significant decline in G6PD in patients of diabetes mellitus with poor glycemic control. Estimation G6PD also can use as screening test in diabetic population to determine their genetic involvement.

Keywords: Type-2 Diabetes Mellitus, HbA1c%, G6PD