Objective: The purpose of this study was to assess the frequency of asymptomatic bacteriuria and causative organisms that are responsible for the development of bacteriuria in pregnant women visiting antenatal checkup in outpatient Department in hospital.

Methodology: This was a Cross sectional study conducted in the Outpatient department of Gynecology, SIMS/Services Hospital, Lahore after taking ethical approval. The duration of the study was about six months. Non-probability purposive sampling technique was used in the study. Any gestational age, all pregnant women with no clinical symptoms of bacteriuria were included in the study. Frequency and percentages was determined for qualitative data like asymptomatic bacteriuria and causative agent (i.e., Staph Aureus, E.coli, Klebsiella pneumonae).

Results: A total of 220 pregnant women were selected. Their mean age were reported 26.12±4.08 years ranging from 17-37 years. Culture and sensitivity results confirmed 16(7.3%) cases with asymptomatic bacteriuria. Among 16 positive cases, E.coli was the predominant organism isolated in 8(50%) samples followed by Staphylococcus aureus in 4(25%) samples, Klebsiella in 1(6.25%), Candida Species in 1 (6.25%) and mixed growth in 2(12.5%) sample were detected in urine culture and sensitivity test.

Conclusion: This study concludes that frequency of asymptomatic bacteriuria was uncommon amongst those pregnant women who attended for antenatal checkup. Most predominant pathogen isolated from culture was Escherichia coli followed by Staphylococcus aureus.

Keywords: Bacteriuria, Escherichia coli, Staphylococcus aureus