Comparing the use of Xylene and Cedarwood oil and its Efficacy in Hematoxylin and Eosin Staining. An Investigational Study
Mohammad Manzoor, Madeha Kushid, Adnan Sarwar, Fatima Daud, Inam-U-Llah, Hina Mir, Muhammad Awais, Sudhair Abbas Bangash
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ABSTRACT
Background:
The use of xylene
as a cleaning agent is required for hematoxylin and eosin staining. On the
other hand, there is cause for concern when it comes to the dangers of xylene
exposure. Xylene has been replaced with a variety of solutions, including
essential oils, during tissue processing. The objective of this research was to see whether Cedarwood oil, an
essential oil, could be utilised as a substitute for Xylene in Hematoxylin and
Eosin staining.
Materials
and Methods: The study was carried out in the Histopathology and Microbiology Department. The department's archives
yielded thirty blocks of paraffin from the regular biopsy material. For my
diffuser combination, I purchased cedar wood oil from an organic and natural
goods store in my neighbourhood. The 30 tissue samples that were processed, each
of the 30 tissue samples that was processed was washed with an essential oil (utilised
8 percent cedarwood oil) or xylene before being sliced into four-micron-thick
slices and stained with E and H stain. They were rated based on the uniformity,
clarity, and transparency of the stained sections.
Results: The three staining quality indicators tested
showed a strong link between cedarwood oil and xylene.
Conclusions: It is our opinion that cedarwood oil may be
used as a xylene substitute in the histopathology laboratory.
Keywords: H and E stain, xylene, histo-techniques,
ABSTRACT
Background:
The use of xylene
as a cleaning agent is required for hematoxylin and eosin staining. On the
other hand, there is cause for concern when it comes to the dangers of xylene
exposure. Xylene has been replaced with a variety of solutions, including
essential oils, during tissue processing. The objective of this research was to see whether Cedarwood oil, an
essential oil, could be utilised as a substitute for Xylene in Hematoxylin and
Eosin staining.
Materials
and Methods: The study was carried out in the Histopathology and Microbiology Department. The department's archives
yielded thirty blocks of paraffin from the regular biopsy material. For my
diffuser combination, I purchased cedar wood oil from an organic and natural
goods store in my neighbourhood. The 30 tissue samples that were processed, each
of the 30 tissue samples that was processed was washed with an essential oil (utilised
8 percent cedarwood oil) or xylene before being sliced into four-micron-thick
slices and stained with E and H stain. They were rated based on the uniformity,
clarity, and transparency of the stained sections.
Results: The three staining quality indicators tested
showed a strong link between cedarwood oil and xylene.
Conclusions: It is our opinion that cedarwood oil may be
used as a xylene substitute in the histopathology laboratory.
Keywords: H and E stain, xylene, histo-techniques,