Expression of P16 in Head and Neck Squamous Cell Carcinoma
Bushra Nasib, Maria Tasneem Khattak, Raazia Mahmood, Shahid Hasnain, Sahibzadi Fatima Tariq, Masroor Hassan
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ABSTRACT
Introduction: Head and neck squamous cell carcinoma (HNSCC)
is a malignant neoplasm
that arises in the oral cavity, nasal
cavity, paranasal sinuses, oropharynx, nasopharnx, and larynx. Among various
prognostic markers in HNSCC, demonstration of p16 protein expression is of
great importance.
Objective:
To determine the frequency of p16
expression in head and neck squamous cell carcinoma by Immuno-histochemistry
(IHC) in patients reporting at Rehman Medical Institute.
Methodology: This was descriptive,
cross sectional study piloted at the Department of Histopathology, Rehman
Medical Institute (RMI) for a period of six months from 10/March/2016 to
10/September/2016. This study was carried out on a total of 103 patients diagnosed
with head and neck squamous cell carcinoma. On Formalin Fixed Parafin embedded
blocks of already diagnosed OSCC cases, IHC staining was done by using p16
(monoclonal) antibody on FFPE blocks of OSCC. Statistical analysis of the data
was done by using the software SPSS version 20.
Results: p16 was positive in 95 cases (92.2%) and
negative in 8 cases (7.8%). Chi-square test was performed between p16 and site
of biopsy, a significant relation was observed with P-value 0.023 (<0.05).
Of all the positive cases, p16 was positive in 51 cases (53.7%) of oral cavity
SCC, 17 cases (17.9%) of pharyngeal SCC, 23 cases (24.2 %) of laryngeal SCC and
4 cases (4.2%) of nasal cavity SCC.
Conclusion: High expression of p16
was observed in HNSCC cases with correlation to various sites of Head and Neck.
ABSTRACT
Introduction: Head and neck squamous cell carcinoma (HNSCC)
is a malignant neoplasm
that arises in the oral cavity, nasal
cavity, paranasal sinuses, oropharynx, nasopharnx, and larynx. Among various
prognostic markers in HNSCC, demonstration of p16 protein expression is of
great importance.
Objective:
To determine the frequency of p16
expression in head and neck squamous cell carcinoma by Immuno-histochemistry
(IHC) in patients reporting at Rehman Medical Institute.
Methodology: This was descriptive,
cross sectional study piloted at the Department of Histopathology, Rehman
Medical Institute (RMI) for a period of six months from 10/March/2016 to
10/September/2016. This study was carried out on a total of 103 patients diagnosed
with head and neck squamous cell carcinoma. On Formalin Fixed Parafin embedded
blocks of already diagnosed OSCC cases, IHC staining was done by using p16
(monoclonal) antibody on FFPE blocks of OSCC. Statistical analysis of the data
was done by using the software SPSS version 20.
Results: p16 was positive in 95 cases (92.2%) and
negative in 8 cases (7.8%). Chi-square test was performed between p16 and site
of biopsy, a significant relation was observed with P-value 0.023 (<0.05).
Of all the positive cases, p16 was positive in 51 cases (53.7%) of oral cavity
SCC, 17 cases (17.9%) of pharyngeal SCC, 23 cases (24.2 %) of laryngeal SCC and
4 cases (4.2%) of nasal cavity SCC.
Conclusion: High expression of p16
was observed in HNSCC cases with correlation to various sites of Head and Neck.