Sickle Cell Disease and Β Globin Gene Haplotypes Among Selected Sudanese Population in North Darfur State
Nassreldeen Khalid Adam,, Maki A, Nahla Ahmed Mohammed Abdurrahman, Mohammed Ahmed Ibrahim Ahmed Khalid Hajissa
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ABSTRACT
Aim: To identify β globin gene haplotypes and
their frequencies in patients with SCD in North Darfur state, western Sudan
Methods: This is a cross-sectional prospective community-based
study that was carried out between December 2017 and August 2018. The study
took place in the North Darfur state which is located in western Sudan. The
study included 666 individuals (369 females and 297 males). Participants
were screened for haemoglobinopathies using haemoglobin electrophoresis, while
β globin haplotypes analysis for patients with SCD was carried out using
polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results: Among the 666 participants, 579 (86.94%) had normal
hemoglobin (AA), while 70 (10.51%), 13 (1.95%), and 4 (0.6%) had AS, SS, and AD
respectively. Cameroon haplotype was found in 42.3% of the study group. Benin
was 26.9%, Bantu was 23.1% and Senegal was 7.7%.
Conclusion: The Cameron haplotype was found to be
most prominent in Sudanese patients, thereby confirming the findings of
previous studies in the country.
Keywords: sickle cell disease;
anemia; β-globin; haplotype; North Darfur; Sudan