ABSTRACT

Background: Wnt signaling pathway is a complex network involving important molecular and functional interaction molecules. It has a key role in the wound healing process.

Aim: To identify the molecular interactions of FZD10 in Wnt signalling pathway in wound healing.

Study Design: Computation based in silico study

Place and Duration of Study: Department of Biochemistry, Swat Medical College (STMC), Saidu Shareef, Swat, Pakistan from 1^st March 2021 to 31^st August 2021.

Methodology: We applied Search Tool for the Retrieval of Interacting Gene/Proteins (STRING) analysis, using specific parameters including (1) Textmining (2) Experiments (3) Databases (4) Co-expression (5) Neighbourhood (6) Gene fusion and (7) Co-occurrence for the identification of molecular interactions of the Wnt signaling pathway. The maximum number of interactors was set at 20 and highest confidence level at 0.900.

Results: STRING network analysis revealed that Frizzled Homologue 10 (FZD10) protein interacts with Hypoxia-Inducible Lipid Droplet-Associated Protein(HILPDA), Wnt1,Wnt3A, Wnt5A,Wnt7A,Wnt7B, Wnt16, Low-density lipoprotein receptor-related protein 5 (LRP5), LRP6, and Dishevelled Segment Polarity Protein 1 (DVL1) proteins in Wnt signaling pathway and contributes in various cellular activities, including cell proliferation, cellular migration, differentiation, apoptosis and stem cell regeneration, which are crucial for the wound healing process.

Conclusion: Functional enrichment analysis revealed that FZD10 protein is a key player in Wnt signaling pathway and has the potential to be considered as a candidate molecule for the therapeutics of wound healing process in future.