Counter effects of Vitamin E against Outcomes of Alcohol Toxicity in Pancreatic Acini: Histomorphometric Study
Noman Ullah Wazir, Jehanzeb Khan, Zilli Huma, Farooq Khan, Nighat Ara, Ambereen Humayun
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ABSTRACT
Aim: To evaluate the effects of alcohol on the
microscopic morphology of the exocrine pancreas and blood serum amylase and to
explored that if vitamin E has a protective role against alcohol-induced damage
in the pancreas of rabbits.
Study
design: Analytical
experimental study
Place
and duration of study: Departments of Anatomy, Pathology
and Pharmacology in Peshawar Medical College Pakistan from 1st
January 2019 to 30th June 2019.
Methodology: Eighteen
healthy adult male domestic rabbits weighing 1-1.5 kg were chosen
(oryctolaguscuniculus). The control group A received proper food and normal
saline as drinking water, experimental group B received proper diet and 30
percent ethanol solution (30ml/kg/day) orally daily with normal saline, and
experimental group C received proper diet, 30 percent ethanol solution
(30ml/kg/day), vitamin E (50mg/kg/day) orally daily with normal saline. Each
rabbit's blood was taken for serum amylase. Morphology of acinar cells
included: 1) number of cells, (10 acini/field, 2) size of acini, 3) size of
acinar cells, and 4) size of acinar nuclei.
Results: Normal
value of serum amylase in rabbits was found. The difference in serum amylase
levels between the control and experimental groups for both E4 and E8 animals
was not statistically significant. There was no significant difference in the
number of pancreatic acinar cells, size of pancreatic acini, the pancreatic
acinar cell size, and pancreatic acinar cells nuclear size in the control and
experimental groups for both E4 and E8 animals.
Conclusion: Alcohol
consumption had no influence on the histomorphology of the rabbits' pancreatic
acini in a short period (4-8 weeks). No significant variation was noted in the
pancreatic acinar cells count & size, pancreatic acinar cells nuclear count
and size, and pancreatic acini size. Therefore, protective role of vitamin E
was not usefully identified.
Keywords: Alcohol,
Pancreas, Histomorphology, Vitamin E, Serum amylase