This study aimed to determine the main species of dermatophytes which caused skin infection and effect of antifungal and Ago and Zno nanoparticles on them. The result of this study showed that out of 80 sample, 54 sample were positive to fungal isolation with ratio 67.5%. and according to culture and PCR results %8383 of isolated type belong to Trichophyton mentagrophytes. Trichophyton mentagrophytes resistant to Nystatin and Fluconazole while sensitive to Griesofulvin, Clotrimazole and Flucytosin. MIC of Ago and Zno nanoparticle against Trichophyton mentagrophytes were 250 and 275 μg /ml while MFC were 275 and 300 μg /ml respectively. Results of RAPD PCR showed that both Ago and Zno nanoparticle effect in genetic material of Trichophyton mentagrophytes

Key words: Trichophyton mentagrophytes, nanoparticle, RAPD PCR