Background: Awareness and knowledge about prevention of infections are important to prevent transmission of diseases from healthcare workers to patients.

Aim: To evaluate the students’ attitude towards infection control at Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia.

Study Design: Cross sectional study.

Methodology: Present study enrolled 415 participants involving both genders from different allied health specialties at PSMCHS. A standardized, anonymous online questionnaire was utilized to gather the data necessary to assess students’ attitudes through answering seven statements. Informed written consent was taken. Unwilling participants were excluded. All collected information was kept confidential. Data analyzed by SPSS 25.0. Frequency and percentage were given for gender distribution and knowledge assessment through questionnaire. Moreover, F- test was applied by keeping CI (95%) with p-value of ≤ 0.05 taken as significant.

Results: There was a significant difference between female and male students regarding the positive attitude towards infection control. Dental and Oral Health students scored the highest degree of agreement using Analysis of Variance (F-test). This high score was obtained because dedicates an entire course infection control while it was embedded in the other departments’ courses.

Conclusion: This study concluded that a dedicated course on infection control was an effective tool in instilling positive attitude towards infection control among PSMCHS students from different specialties.

Keywords: Attitude, Awareness, Infection control and Students.