Naila Noor, Arsalan Ahmed Uqaili, Keenjher Rani, Salahuddin Shaikh,Navaid Kazi, Farzana Rahim

Evaluation of Protective Role of Lutein Against Ribavirin Induced RBC Membrane Damage in Rat Model

Naila Noor, Arsalan Ahmed Uqaili, Keenjher Rani, Salahuddin Shaikh,Navaid Kazi, Farzana Rahim



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ABSTRACT

Objective: To determine the protective role of lutein against RBC cell membrane damage assessed by RBC osmofragility test, Peripheral blood smear and blood CP.

Subjects and Methods:Present experimental study was carried out at Isra University, Hyderabad and Department of AHVS (Animal Husbandry) Agriculture University,Tando Jam. 30 albino Wistar rats was selected and were divided into 3 groups namely A (controls), and experimental groups B which was given ribavirin only for 4 weeks and Group C was given Ribavirin as inducing agent and lutein. All drugs were used for 4 weeks as an oral dose daily. Erythrocyte osmotic fragility test was performed with NaCl solutions of difference osmotic concentrations. Data was analyzed by SPSS version 21.

Results:Hemolysis was higher 81% in group B as compared to group C as 70%. Mean of RBC count in the groups B was markedly decreased as compared to group A and C4.65, 3.29, and 5.13 (P=0.036). Mean of MCV in the grops A,B,C was noted as 53.4, 37.3 and 55.6 respectively (P=0.0001). Mean of MCH in the groups A,B,Cwas notedas 19.4, 18.7,and19.1 respectively (P=0.09), while mean of HCT in the groups A,B,C was noted as 34.2 , 25.7 and 34.2 (P=0.004)

Conclusion: It was concluded that lutein have protective role against ribavirin induced hemolysis in RBCs of rats

Keywords: Ribavirin, Hemolysis, Lutein



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