ABSTRACT

The aim of the study is to investigate purified protease produced by Acinetobacter baumannii as an antibacterial agent against several gram-positive and gram-negative pathogenic bacteria. In this study, two hundred-thirty samples were collected from many hospitals in Baghdad from September 2019 to December 2019; Specimens were collected from numerous sources. Out of 230 clinical specimens, 50 (21.7%) were identified as Acinetobacter baumannii which was isolated from C.S, F 18(36%); blood specimens constituted 15(30%); burn and wounds specimens formed 10(20%); sputum specimens achieved 7(14%). A total of 50 isolates were used in screening for protease production on skim milk agar, 30/50 isolates being positive for protease by forming a clear zone around the colonies in different degrees. After that, the strongest protease producer A. baumannii was taken to study the optimum condition for its production and purification protease.

Keywords: Antibacterial, Purified Protease, Acinetobacter baumannii