ABSTRACT

Background: Arsenic contamination among heavy metals in ground water is a health hazard due to its toxicological concern, affecting millions of humans globally.

Aim: To observe hepato-protective effect of vitamin-E on sodium arsenite induced microscopic hepatic changes among albino rats.

Study Design: Cross Sectional study.

Methodology: 90 adult albino male rats (30 in each group), weighing 140 to 180 grams were taken. They were kept under controlled temperature of 23+2 and humidity 50+5% in the experimental research lab of postgraduate medical institute Lahore. The night and day cycle was maintained for 12 hours each. Each animal of every group was labeled from 1-30 with different color for every group. Standard diet and tap water and libitum was given Results: The initial and final body weights of animals in all the three study groups were shown in table-1 as mean ± SD. There was significant difference among three groups as regard to fatty degeneration of hepatocytes with p <0.001. Significant difference was noted among each group as regard to pyknosis. The difference among groups for number of inflammatory cells was significant with p-value <0.001.

Conclusion: Vitamin-E can be employed as anti-oxidant agent for treatment against arsenic induced hepatoxicity. It improves microscopic changes in lever hence its can be used as hepato-protective agent.

Keywords: Vitamin-E, Hepato-protection, Relative Tissue Weight Index and Albino Rats.