Cytotoxicity A549 Cancer Cells Apoptotic - Effect of GW9508 Cytotoxicity on A549 Cancer Cells and Expression Bax Apoptotic Gene
Sima Orouei, Maliheh Entezari, Mehrdad Hashemi, Kiavash Hushmandi, Abolfazl Movafagh
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ABSTRACT
Background: Small molecules such as GW9508 can easily
cross the cell membrane and reach the target area because of their very low
weight. Also, due to the high costs of treatment and cancer drugs, the need to
replace pharmacologic factors and cost-effective anticancer drugs is felt.
Aim: To investigate the
anticancer and cytotoxic effects of GW9508 on A549 lung cancer cells.
Methods: In this experiment, cell line (A549)
was first cultured in DMEM medium containing 10% FBS and then treated with
different concentrations of both compounds. MTT assay was performed on days 1,
3 and 5 to determine IC50 and to compare the viability of cells treated with
different concentrations of GW9508. QRT-PCR assay was used to evaluate the
effect of GW9508 with IC50 concentration on apoptosis induction and Bax gene
expression.
Results: The results showed that GW9508 in a
dose and time-dependent manner significantly reduced the viability and
proliferation of A549 cells (P≤0.001). The control group showed a significant
decrease compared to the first- and third-day samples (P <0.05).
Morphological changes such as decreased chromatin density and cell rounding
were also observed in the cells. Also, molecular results showed that GW9508 was
able to increase Bax gene expression.
Conclusion: GW9508 small molecule induces cell
death in lung cancer cells by decreasing cell viability and increasing Bax gene
expression. As a result, it has the potential to induce cell death in cancer
cells and cancer treatment.
Keywords: GW9508, Lung Cancer, Apoptosis
ABSTRACT
Background: Small molecules such as GW9508 can easily
cross the cell membrane and reach the target area because of their very low
weight. Also, due to the high costs of treatment and cancer drugs, the need to
replace pharmacologic factors and cost-effective anticancer drugs is felt.
Aim: To investigate the
anticancer and cytotoxic effects of GW9508 on A549 lung cancer cells.
Methods: In this experiment, cell line (A549)
was first cultured in DMEM medium containing 10% FBS and then treated with
different concentrations of both compounds. MTT assay was performed on days 1,
3 and 5 to determine IC50 and to compare the viability of cells treated with
different concentrations of GW9508. QRT-PCR assay was used to evaluate the
effect of GW9508 with IC50 concentration on apoptosis induction and Bax gene
expression.
Results: The results showed that GW9508 in a
dose and time-dependent manner significantly reduced the viability and
proliferation of A549 cells (P≤0.001). The control group showed a significant
decrease compared to the first- and third-day samples (P <0.05).
Morphological changes such as decreased chromatin density and cell rounding
were also observed in the cells. Also, molecular results showed that GW9508 was
able to increase Bax gene expression.
Conclusion: GW9508 small molecule induces cell
death in lung cancer cells by decreasing cell viability and increasing Bax gene
expression. As a result, it has the potential to induce cell death in cancer
cells and cancer treatment.
Keywords: GW9508, Lung Cancer, Apoptosis