ABSTRACT

Background: Dugs formed from animals are in common use today for their effective results in treatment.

Aim: The goal was to appraise hepato-renal protective effect Dinothrombium tacnitorium methanolic extract in Albino rats on their histological basis.

Study design: It was a randomised control study.

Methodology: Aqueous methanolic extract (70% v/v) of Dinothrombium tacnitorium (Dt.Cr) was prepared followed by succeeding evaporation using rotary evaporator. Liver and renal toxicity was done by introduction of CCl₄ (2 ml/kg, p.o) in paraffin oil on 7^th day of experiment. By the end of project, liver and kidneys were taken out under anesthesia. They were sectioned into 5 µm by microtome. They were stained with Eosin-Hematoxylin dye. They were observed for histo-pathological changes. Administration of methanolic extract of Dinothrombium tacnitorium (300mg/kg body weight/day) orally protected against the CCl₄ induced elevation of liver enzymes as well as renal serum markers. All hepatic enzymes and renal markers were elevated in the CCl₄ alone treated animals. Administration of methanolic extract to CCl₄ challenge protected against the hepato-renal toxic agent.

Conclusion: Histological findings suggested that the red velvet mite methanolic extract reduces the histo-pathological changes in both liver and kidneys due to CCl₄-induced toxicity in albino rats thus it can work as hepato-renal protective agent.

Key words: Dinothrombium tacnitorium, Carbon-tetrachloride, hepatic & renal markers, Albino Rats and Histology.